Microencapsulation of Bacillus subtilis E20 Probiotic, a Promising Approach for the Enrichment of Intestinal Microbiome in White Shrimp, Penaeus vannamei

Microencapsulation is an advanced technique used to improve the viability of probiotics and minimize sensitivity during processing, storage, in gastrointestinal environment. Two dietary treatments including a control encapsulated probiotic, Bacillus subtilis E20 (EP), were evaluate efficacy improving intestinal microbiome white shrimp, Penaeus vannamei, after 60-feeding trial. The 16S rDNA next-generation sequencing (NGS) analysis indicated that shrimp fed EP diet generated higher amplicon reads than diet. No significant differences observed α-diversity index microbiota At phylum level, Proteobacteria was relatively abundant both treatment with increased expression Tenericutes, Bacteroidetes, Firmicutes, more control. PC revealed altered bacterial profile shrimp’s intestines into forming different clusters. Unique genera such as Luteolibacter, Simkaniaceae, Haemophilus, Pirellulaceae, Filomicrobium, Sphingomonas, Erysipelotrichaceae UCG-003 along well-known probiotic Lactobacillus found intestine PCA eigenvector plots abundance diet, but Vibrio These results suggest B. can be beneficial microbiota.